The growth and differentiation of mesenchymal stem and progenitor cells cultured on aligned collagen matrices.

摘要: Cell-matrix interactions are paramount for the successful repair and regeneration of damaged diseased tissue. Since many tissues have an anisotropic architecture, it has been proposed that aligned extracellular matrix (ECM) structures in particular could guide support differentiation resident mesenchymal stem progenitor cells (MSCs). We therefore created collagen type I using a microfluidic set-up with aim to assess their impact on MSC growth differentiation. In addition, we refined our matrices by incorporating glycosaminoglycan (GAG) heparin demonstrate versatility applied methodology study multiple ECM components single system. Our reconstituted, maintained allowed multilineage (osteogenic/adipogenic/chondrogenic) MSCs. Most noticeable was observation during osteogenesis, substrates choreographed ordered mineralization. Likewise, myotube assembly C2C12 profoundly influenced topographic features resulting enhanced organization length. results shed light regulation MSCs through directional these cell culture platforms guiding morphogenesis tissue types structures.