Recombinant Production of Native Proteins from Escherichia coli
作者： Tsutomu Arakawa , Tiansheng Li , Linda O. Narhi
DOI: 10.1007/978-1-4615-0557-0_2
关键词:
摘要: The production of large quantities proteins became possible with the advent recombinant DNA technology, and subsequent expression in Escherichia coli (E. coli) (Itakura, 1977). Recombinant are produced E. cytoplasm at high concentrations a very different environment than that which they normally expressed. Their structure, stability solubility affected by this can be quite native protein. Therefore, purification often requires techniques those used to purify same protein from its natural environment. Numerous attempts have been made and/or refold expressed proteins.
springer.com
sci-hub.st 参考文章(145)
Mark Ratner, Genentech: Don't Sell the Company Short Nature Biotechnology. ,vol. 7, pp. 114- 114 ,(1989) , 10.1038/NBT0289-114
K. Nagahari, S. Kanaya, K. Munakata, Y. Aoyagi, S. Mizushima, Secretion into the culture medium of a foreign gene product from Escherichia coli: use of the ompF gene for secretion of human beta-endorphin. The EMBO Journal. ,vol. 4, pp. 3589- 3592 ,(1985) , 10.1002/J.1460-2075.1985.TB04121.X
Satish K. Sharma, Ramnath Seetharam, Purification and Analysis of Recombinant Proteins ,(1991)
Protein folding : in vivo and in vitro American Chemical Society. ,(1993) , 10.1021/BK-1993-0526
T Kudo, C Kato, K Horikoshi, Excretion of the penicillinase of an alkalophilic Bacillus sp. through the Escherichia coli outer membrane. Journal of Bacteriology. ,vol. 156, pp. 949- 951 ,(1983) , 10.1128/JB.156.2.949-951.1983
C H Chung, H E Ives, S Almeda, A L Goldberg, Purification from Escherichia coli of a periplasmic protein that is a potent inhibitor of pancreatic proteases. Journal of Biological Chemistry. ,vol. 258, pp. 11032- 11038 ,(1983) , 10.1016/S0021-9258(17)44382-1
G M Fox, S G Schiffer, M F Rohde, L B Tsai, A R Banks, T Arakawa, Production, biological activity, and structure of recombinant basic fibroblast growth factor and an analog with cysteine replaced by serine. Journal of Biological Chemistry. ,vol. 263, pp. 18452- 18458 ,(1988) , 10.1016/S0021-9258(19)81379-0
Susan A. Charman, Kaye L. Mason, William N. Charman, Techniques for assessing the effects of pharmaceutical excipients on the aggregation of porcine growth hormone. Pharmaceutical Research. ,vol. 10, pp. 954- 962 ,(1993) , 10.1023/A:1018994102218
Alan M. Krensky, Peter J. Nelson, Expression of chemokine RANTES and production of monoclonal antibodies. Methods in Enzymology. ,vol. 287, pp. 162- 174 ,(1997) , 10.1016/S0076-6879(97)87013-9
T Arakawa, N K Alton, Y R Hsu, Preparation and characterization of recombinant DNA-derived human interferon-gamma. Journal of Biological Chemistry. ,vol. 260, pp. 14435- 14439 ,(1985) , 10.1016/S0021-9258(17)38587-3