Preparation and characterization of recombinant DNA-derived human interferon-gamma.
作者： T Arakawa , N K Alton , Y R Hsu
DOI: 10.1016/S0021-9258(17)38587-3
关键词:
摘要: An attempt was made to prepare a highly purified, active recombinant DNA-derived human interferon-gamma. When the protein denatured in urea and refolded, gel filtration sedimentation velocity experiments indicated presence of two forms, which are different size not rapid reversible equilibrium. The forms could be chromatographically separated. Far-UV circular dichroic spectra secondary structures for both forms. Near-UV revealed that smaller form is folded into rigid tertiary structure. antiviral activity interferon-gamma showed significant difference, i.e. 4-8-fold more than larger form. A variety show active, homogeneous, soluble, stable
sci-hub.st 参考文章(17)
Sidney Pestka, Samuel Baron, [1] Definition and classification of the interferons Methods in Enzymology. ,vol. 78, pp. 3- 14 ,(1981) , 10.1016/0076-6879(81)78091-1
Serge N. Timasheff, 8 Some Physical Probes of Enzyme Structure in Solution The Enzymes. ,vol. 2, pp. 371- 443 ,(1970) , 10.1016/S1874-6047(08)60187-0
H C Kelker, J Le, B Y Rubin, Y K Yip, C Nagler, J Vilcek, Three molecular weight forms of natural human interferon-gamma revealed by immunoprecipitation with monoclonal antibody. Journal of Biological Chemistry. ,vol. 259, pp. 4301- 4304 ,(1984) , 10.1016/S0021-9258(17)43045-6
S Pestka, B Kelder, P C Familletti, J A Moschera, R Crowl, E S Kempner, Molecular weight of the functional unit of human leukocyte, fibroblast, and immune interferons. Journal of Biological Chemistry. ,vol. 258, pp. 9706- 9709 ,(1983) , 10.1016/S0021-9258(17)44554-6
E Rinderknecht, B H O'Connor, H Rodriguez, Natural human interferon-gamma. Complete amino acid sequence and determination of sites of glycosylation. Journal of Biological Chemistry. ,vol. 259, pp. 6790- 6797 ,(1984) , 10.1016/S0021-9258(17)39797-1
H C Kelker, Y K Yip, P Anderson, J Vilcek, Effects of glycosidase treatment on the physicochemical properties and biological activity of human interferon-gamma. Journal of Biological Chemistry. ,vol. 258, pp. 8010- 8013 ,(1983) , 10.1016/S0021-9258(20)82020-1
Patrick W. Gray, David W. Leung, Diane Pennica, Elizabeth Yelverton, Richard Najarian, Christian C. Simonsen, Rik Derynck, Pamela J. Sherwood, Donald M. Wallace, Shelby L. Berger, Arthur D. Levinson, David V. Goeddel, Expression of human immune interferon cDNA in E. coli and monkey cells Nature. ,vol. 295, pp. 503- 508 ,(1982) , 10.1038/295503A0
Andrzej Galat, Thomas A. Creighton, Richard C. Lord, Elkan R. Blout, Circular dichroism, Raman spectroscopy, and gel filtration of trapped folding intermediates of ribonuclease. Biochemistry. ,vol. 20, pp. 594- 601 ,(1981) , 10.1021/BI00506A023
Lois B. Epstein, Martin J. Cline, Thomas C. Merigan, PPD-stimulated interferon: in vitro macrophage-lymphocyte interaction in the production of a mediator of cellular immunity. Cellular Immunology. ,vol. 2, pp. 602- 613 ,(1971) , 10.1016/0008-8749(71)90008-6
Y. K. Yip, R. H. Pang, C. Urban, J. Vilcek, Partial purification and characterization of human gamma (immune) interferon Proceedings of the National Academy of Sciences of the United States of America. ,vol. 78, pp. 1601- 1605 ,(1981) , 10.1073/PNAS.78.3.1601