Engineering Rhodosporidium toruloides with a membrane transporter facilitates production and separation of carotenoids and lipids in a bi-phasic culture.

摘要: The oleaginous yeast Rhodosporidium toruloides has great biotechnological potential. It accumulates a high amount of lipids which can be used for biofuels and also produces carotenoids are valuable in the food pharmaceutical industry. However, location these two hydrophobic products cell membrane prohibits its efficient harvesting separation. Here, transporter Pdr10 was engineered into R. cultured two-phase media containing oil. This enabled production situ export oil concurrent separation from intracellular cells. When strain media, fatty acids yield increased 1.9 to 2.9 μg/mg 0.07 0.09 mg/mg, respectively. A total 1.8 exported by strain, as compared 0.3 wild type. In composition acid it produced changed. Torulene became major carotene instead torularhodin. Also, unsaturated C18:2 dominant saturated C16:0, similar grape seed media. indicated that being consumed cells, supported glycerol levels detected gas chromatography-mass spectrometry (GC-MS). Our approach represents an easy greener extraction method could serve increase facilitate acids.