Model Experiments for the Study of Iron Transfer from Transferrin to Ferritin

摘要: 1 The transfer of iron from transferrin to ferritin was followed using radioactive tracers. It a passive process which unaffected by the moving against concentration gradient. 2 A reducing agent essential and had three functions:–(a) assist in removal transferrin; (b) form ferrous chelate as an intermediate; (c) ensure that is for uptake on ferritin. Ascorbic acid best those agents tested. 3 Transfer enhanced chelators. The formed must be stable enough effect transferrin, but not so prevent formation complex. 4 Increasing concentrations chelator, or all enhance percentage transfer. 5 The reaction pH dependant at maximum 7.3. 6 Transfer content used. Transferrin with 50% less saturation does iron. Ferritin takes up most when it about saturated. 7 The α, β γ fractions were isolated their order found > α γ.