The transition from proliferation to differentiation in colorectal cancer is regulated by the calcium activated chloride channel A1

摘要: Breaking the balance between proliferation and differentiation in animal cells can lead to cancer, but mechanisms maintaining this remain largely undefined. The calcium activated chloride channel A1 (CLCA1) is a member of sensitive conductance family proteins expressed mainly colon, small intestine appendix. We show that CLCA1 plays functional role Caco-2 intestinal tissue. spontaneously differentiate either confluent culture or when treated with butyrate, molecule present naturally diet. Here, we compared expressional levels patients without colorectal cancer (CRC) determined cells. showed that: 1) CLCA4 expression were down-regulated significantly CRC patients; 2) was up-regulated induced by treatment sodium butyrate (NaBT); 3) Knockdown siRNA inhibited cell promoted cultures, 4) In 3D culture, suppression increased compromised NaBT-induced inhibition proliferation. conclusion, may contribute promoting spontaneous reducing be target therefore potential diagnostic marker for prognosis.