Validation of tissue microarray biomarker expression of breast carcinomas in Saudi women

作者: Abdulmohsen Alkushi

DOI: 10.1016/S1658-3876(09)50007-6

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摘要: BACKGROUND Analysis of immunohistochemical expression a large number tumor tissue samples with conventional techniques is costly, tedious and slow. Tissue microarray (TMA) technology can facilitate the sampling over 500 tumors on one-glass slide, which then be analyzed by fluorescence in situ hybridization (FISH), RNA hybridization, or immunohistochemistry. We attempted to validate this technique breast cancer specimens comparing staining result obtained TMA whole-section method. PATIENTS AND METHODS Eighty cases diverse subtypes were used build biomarker evaluation model. Serial sections recipient block immunostained panel 4 antibodies (ER, PR, HER2 p53). Concordance between whole-sections was expressed as κ statistic. RESULTS Target accurately sampled two cores 19 26 donor blocks, only one core 5 blocks. Failure sample seen 2 results whole good for PR (κ = 0.67) ER very p53 (κ = 0.91) (κ = 0.91), when all blocks included. The rate improved excellent (κ = 1.0) did not change other markers concordance analysis limited that had been cores. CONCLUSION reliable examining set tumors. It shows immunostaining scores comparable those cancer. However, some alterations are detected due heterogeneity inherent these tumors, but shortfall improved.

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