MDR1 gene expression in primary and advanced breast cancer.

摘要: P-glycoprotein (Pgp)-associated multidrug resistance (MDR) is related to intrinsic and acquired cross anthracyclines, vinca alkaloids, other antineoplastic antibiotics. Expression of MDR1 widely considered play an important role in conferring adjuvant chemotherapy women with breast tumor cells disseminated disease, although data supporting this view is, at best, conflicting. The expression gene its product, P-glycoprotein, was investigated primary advanced cancers (both previously untreated treated on specific treatment protocols) assess the determining responsiveness chemotherapy. assessed by immunohistochemistry, reverse transcription-PCR (RT-PCR), Northern Blot Western Blot. mRNA detected 40% tested RT-PCR 40 cycles PCR amplification. When reducing amplification 28, signal disappeared from highest expressers; however, known positive control normal tissues, such as adrenal, kidney, liver continued show product. blots failed demonstrate these cancers. In contrast, physiologic levels clearly techniques. Immunohistochemistry confirmed that carcinoma lacked expression; interstitial mononuclear cells, morphologically consistent lymphocytes or macrophages did immunostaining some tumors. identified not correlated either response paclitaxel therapy (29 patients able be evaluated, p = 0.34, Fisher Exact Test) overall survival (32 cancer clinical follow-up information, 0.336, log rank). conclusion, expressed significant levels, it stomal macrophages. These results suggest does a cancer.