Transcriptional Activation of Estrogen Receptor α in Human Breast Cancer Cells by Histone Deacetylase Inhibition

摘要: Recent findings have established a connection between DNA methylation and transcriptionally inactive chromatin characterized by deacetylated histones. Because the absence of estrogen receptor α (ERα) gene expression has been associated with aberrant its CpG island in significant fraction of breast cancers, we tested whether histone deacetylase activity contributes to transcriptional inactivation methylated ER panel ER-negative human breast cancer cells. Treatment these cells with trichostatin A, specific histone deacetylase inhibitor, led dose- and time-dependent re-expression of ER mRNA as detected reverse transcription-PCR without alteration in ERα methylation. Trichostatin A-induced ER re-expression was increased sensitivity DNase I at the ER locus MDA-MB-231 cells. These data implicate inactive mediated deacetylation critical component silencing Therefore, may be potential target for therapeutic intervention treatment subset ER-negative breast cancers.