Bacillus megaterium neutral protease, a zinc-containing metalloenzyme
作者： L. Keay , J. Feder , L.R. Garrett , M.H. Moseley , N. Cirulis
DOI: 10.1016/0005-2795(71)90302-3
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摘要: Abstract 1. 1.| Bacillus megaterium strain MA protease has been examined to determine its relationship other well characterized proteases. 2. 2.| B. purified by treatment with active charcoal, (NH 4 ) 2 SO precipitation and hydroxylapatite chromatography. 3. 3.|The shown be a zinc-containing neutral one zinc atom per 40 000 molecular weight. 4. 4.|The specificity against series of dipeptide substrates is similar (although quantitatively different) that subtilis thermolysin. 5. 5.|The partially crossreacts anti-thermolysin serum loss enzyme activity accompanying the precipitin reaction.
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