Photodynamic therapy with two different photosensitizers as a supplement to instrumentation/irrigation procedures in promoting intracanal reduction of Enterococcus faecalis.

摘要: Introduction: This in vitro study aimed to investigate the antibacterial effects of photodynamic therapy (PDT) with methylene blue (MB) or toluidine (TB) (both at 15mg/mL) as a supplement instrumentation/irrigation root canals experimentally contaminated Enterococcus faecalis. Methods: Seventy extracted teeth had their an endodontic strain E. faecalis for 7 days, instrumented nickel-titanium instruments and irrigated either 2.5% NaOCl 0.85% NaCl, then randomly distributed into four experimental groups: MB/NaOCl (PDT MB irrigant), TB/NaOCl TB MB/NaCl NaCl TB/NaCl irrigant). For PDT, photosensitizer remained canal 2 minutesbeforeexposedtoredlightemittedfromadiode laserfor4minutes.Samplesweretakenbeforeandafter following specific PDTprocedureforeachgroup,platedontoMitis-salivarius agar colony forming units counted. Results: Regardless irrigant used (NaOCl NaCl), instrumentation significantly reduced bacterial counts comparison baseline (p 0.05). No significant differences were observed between two photosensitizers > Conclusion: These results suggest that PDT may not exert supplemental effect procedures regard intracanal disinfection. Further adjustments inthe protocolmay be required enhance predictability inbacterial elimination before clinicaluse isrecommended.(JEndod 2009;-:1‐5)