Pulsed electric field permeabilization and extraction of phycoerythrin from Porphyridium cruentum

摘要: Abstract This paper assesses the extraction of β-phycoerythrin (BPE) into aqueous media by application pulsed electric field treatments (PEF) to fresh biomass Porphyridium cruentum. An increase in strength from 2 10 kV/cm, or a prolongation treatment time increased electroporation and inactivation P. cruentum range investigated (2–10 kV/cm; 30–150 μs). A perfect agreement between fraction dead cells electroporated was observed when more than 20% were inactivated. Even after 48 h incubation, BPE not detected medium containing untreated cruentum: thus, an intact cytoplasmic membrane prevented exit BPE. After 24 h extraction, entire content (32 mg/g d.w.) released treating at 8 10 kV/cm for 150 μs. However, immediately PEF treatment, thereby requiring, most cases, lag over 6 h until compound could be medium. behavior indicates that requires only diffusion across cell membrane, but also dissociation structures. In this sense, it is hypothesized autolysis triggering main cause involved effectivity these extraction. improved grasp kinetics mechanism enzymes participating microalgae autolysis, trigger PEF, will allow process developed industrial scale.