Gene transfer with liposomes to the intraocular tissues by different routes of administration.

摘要: Purpose. To determine whether a reporter gene carried by liposomes can be introduced into the ocular tissues in vivo different routes of administration. Methods. Three kinds carrying plasmid DNA with beta-galactosidase were applied topically to eye or injected anterior chamber, subretinal space, and vitreous adult Wistar rats. Gene expression was detected enzymatic color reaction using X-gal as substrate enucleated eyes 1 day, week, month after topical application injection. Results. Topical could transfer retinal ganglion cells. Injection chamber delivered basal layer corneal epithelium, ciliary stroma body iris, space resulted cells, pigment epithelial Conclusions. Efficient stable functional achieved cornea, body, retina Liposomes appear promising vehicle for delivering therapeutic genes mammalian intraocular tissues.