Human blood MAIT cell subsets defined using MR1 tetramers.

摘要: Mucosal-associated invariant T (MAIT) cells represent up to 10% of circulating human T-cells. They are usually defined using combinations non-lineage-specific (surrogate) markers such as anti-TRAV1-2, CD161, IL-18Rα and CD26. The development MR1-Ag tetramers now permits the specific identification MAIT based on TCR specificity. Here, we compare these approaches for identifying show that surrogate not always accurate in cells, particularly CD4fraction. Moreover, while all cell subsets produced comparable levels IFNγ, TNF IL-17A, CD4population more IL-2 than other subsets. In a ontogeny study, frequencies most MR1 tetramerMAIT with exception CD4MAIT increased from birth about 25 years-of-age declined thereafter. We also demonstrate positive association between frequency unconventional including Natural Killer (NKT) Vδ2γδ cells. Accordingly, this study demonstrates phenotypically functionally diverse, may reliably identify their numbers regulated an age-dependent manner correlate NKT This article is protected by copyright. All rights reserved.