Tumour-infiltrating CD8+ lymphocytes and colorectal cancer recurrence by tumour and nodal stage.

摘要: Intratumoural T-cell infiltrate intensity cortes wrelaith clinical outcome in stage II/III colorectal cancer (CRC). We aimed to determine whether this association varies across heterogeneous group. performed a pooled analysis of 1804 CRCs from the QUASAR2 and VICTOR trials. CD8+ CD3+ densities were quantified by immunohistochemistry tissue microarray (TMA) cores, their with analysed Cox regression. validated our results using publicly available gene expression data 1375 seven independent series. In QUASAR2, intratumoural was stronger predictor CRC recurrence than showed similar discriminative ability both markers combination. Pooled multivariable trials increasing density associated reduced risk confounders including DNA mismatch repair deficiency, POLE mutation chromosomal instability (multivariable hazard ratio [HR] for each two-fold increase = 0.92, 95%CI = 0.87–0.97, P = 3.6 × 10−3). This not uniform strata defined tumour nodal stage: absent low-risk (pT3,N0) cases (HR = 1.03, 95%CI = 0.87–1.21, P = 0.75), modest intermediate-risk (pT4,N0 or pT1-3,N1-2) (HR = 0.92, 95%CI = 0.86–1.0, P = 0.046) strong high-risk (pT4,N1-2) (HR = 0.87, 95%CI = 0.79–0.97, P = 9.4 × 10−3); PINTERACTION = 0.090. Analysis CD8A validation cohort revealed variation prognostic value (PINTERACTION = 0.048). The cell infiltration strata.