Temporal renal expression of angiogenic growth factors and their receptors in experimental diabetes: role of the renin-angiotensin system

摘要: Objective: It has been postulated that vascular endothelial growth factor (VEGF) plays a role in the progression of renal injury. However, other angiogenic factors and their receptors, such as angiopoietins Tie2, particular relation to renoprotective therapies, agents interrupt renin-angiotensin system, have not studied context diabetes-related Design: methods Renal expression VEGF, angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2) VEGF-R2 Tie-2, were assessed using reverse transcription-polymerase chain reaction, immunohistochemistry Western blotting, control streptozotocin diabetic rats, untreated or receiving AT receptor antagonist, valsartan, PD123319. Results: Diabetes was associated with increased gene protein VEGF-R2, Ang-1, Ang-2 Tie-2. antagonism attenuated these cytokines yet PD123319, which had no effect on blood pressure, reduced Ang-1 decreased levels. Conclusions: In experimental diabetes, there is significant upregulatlon within kidney various receptors. Furthermore, effects angiotensin II blockade parameters consistent VEGF-VEGF-R2 angiopoietin - Tie-2 axes being modulated by haemodynamic context.