Inhibition of GABA uptake in the rat hippocampal slice

摘要: Pharmacological manipulations known to inhibit GABA uptake prolonged GABA-evoked conductance increases in CA1 pyramidal cells the rat hippocampal slice preparation. Treatments included reduction of extracellular sodium and exposure cis-4-OH-nipecotic acid, nipecotic acid or l -2,4-diaminobutyric (all at 1 mM). These effects contrast with results obtained 4-OH-iso-nipecotic an inactive structural analog which had no effect on time-course responses. 4,5,6,7-Tetrahydroisoxazolo[4,5-c]pyridine-3-ol (THPO), impotent but selective inhibitor into glia, did not prolong The depended distance between source its receptors, as predicted for uptake-limited response. GABA-receptor agonists that are poor substrates (muscimol, thiomuscimol, piperidine-4-sulphonic isoguvacine 4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridine-3-ol (THIP) evoked very long changes were further by inhibitors. demonstrate presence a functional system slice. accessibility GABAergic synapses susceptibility hippocampus epileptiform events suggest could be valuable CNS preparation study role synaptic physiology.