Nitric oxide signaling: a possible role for G proteins.

摘要: We have previously reported various inductive effects of nitric oxide on human PBMC. describe a novel and potentially important mechanism signaling-through direct activation guanine nucleotide-binding proteins (G proteins). found that treatment membranes isolated from fresh PBMC enhances the ability these to hydrolyze [gamma-32P]GTP bind [gamma-35S]GTP. In addition, whole cells with yielded enhanced GTPase activity. Furthermore, activity pure, recombinant Gs alpha, Gi alpha 1, p21ras was greatly by oxide. support existence this pathway in cells, we G protein inhibitor, GDP-beta-S, blocked NF-kappa B translocation induced or LPS permeabilized cells. reduced pertussis toxin-mediated ADP-ribosylation 45- 41-kDa Because play central role many diverse signaling systems, an endogenous inducible oxidant may represent pathway.