Myristic acid is attached to the transforming protein of Rous sarcoma virus during or immediately after synthesis and is present in both soluble and membrane-bound forms of the protein.

摘要: Myristic acid, a minor component of cellular fatty acids, has been shown previously to be covalently bound most molecules p60src, the transforming protein Rous sarcoma virus. We have now determined at what time during life cycle and where within cell, this lipid becomes attached protein. p60src was found acquire myristic acid only one time, or immediately after its synthesis. is known synthesized on free polysomes appears cytoplasmic face plasma membrane lag 10 min. The addition therefore precedes binding membrane. permanent, metabolically stable part protein; we no evidence for turnover myristyl moiety. However, did find myristate various soluble forms large number cytosolic proteins as well. This demonstrates that attachment not in itself sufficient convert into membrane-bound