A truncated heavy chain protein relieves the requirement for surrogate light chains in early B cell development.

摘要: Early B cell development depends upon the surface expression of Ig heavy chain protein (mu) in a signaling complex known as pre-B receptor (pre-BCR). In addition to mu, pre-BCR consists surrogate light chains VpreB and lambda5 transmembrane signal transduction proteins Ig-alpha Ig-beta. Expression this is associated with changes marker expression, gene transcription, rearrangement. Mutations preventing either mu or result developmental arrest, but precise roles various components remain unclear. Using mice transgenic for surface-expressed, truncated, form that cannot associate chains, we have studied role development. We found truncated transgene resulted germline kappa locus V(D)J recombinase targeting indistinguishable from those induced by intact protein. These experiments lead us conclude while necessary assembly wild-type pre-BCR, are not directly involved otherwise required early