Influence of complex nutrients, temperature and pH on bacteriocin production by Lactobacillus sakei CCUG 42687.

作者: I. M. Aasen , T. Møretrø , T. Katla , L. Axelsson , I. Storrø

DOI: 10.1007/S002530050003

关键词:

摘要: The effects of process conditions and growth kinetics on the production bacteriocin sakacin P by Lactobacillus sakei CCUG 42687 have been studied in pH-controlled fermentations. fermentations could be divided into phases based kinetics, phase one being a short period exponential growth, three subsequent ones with decreasing specific rate. Sakacin was maximal at 20 °C. At higher temperatures (25–30 °C) ceased lower cell masses, when less glucose consumed, resulting much concentrations. With similar media pH, maximum concentration °C seven times than that 30 rate increased increasing concentrations yeast extract, concomitantly. Increasing tryptone also had positive influence upon production, though effect significantly extract. obtained this study 20.5 mg l−1. On basis possible metabolic regulation synthesis is discussed, e.g. availability essential amino acids, other nutrients, energy.

参考文章(23)
Luc De Vuyst, Erick J. Vandamme, Lactic Acid Bacteria and Bacteriocins: Their Practical Importance Springer, Boston, MA. pp. 1- 11 ,(1994) , 10.1007/978-1-4615-2668-1_1
Lars Axelsson, Tone Katla, Merete Bj�rnslett, Vincent G.H Eijsink, Askild Holck, A system for heterologous expression of bacteriocins in Lactobacillus sake Fems Microbiology Letters. ,vol. 168, pp. 137- 143 ,(1998) , 10.1111/J.1574-6968.1998.TB13266.X
F. Krier, A. M. Revol-Junelles, P. Germain, Influence of temperature and pH on production of two bacteriocins by Leuconostoc mesenteroides subsp. mesenteroides FR52 during batch fermentation. Applied Microbiology and Biotechnology. ,vol. 50, pp. 359- 363 ,(1998) , 10.1007/S002530051304
O. T. Skjerdal, H. Sletta, S. G. Flenstad, K. D. Josefsen, D. W. Levine, T. E. Ellingsen, Changes in intracellular composition in response to hyperosmotic stress of NaCl, sucrose or glutamic acid in Brevibacterium lactofermentum and Corynebacterium glutamicum Applied Microbiology and Biotechnology. ,vol. 44, pp. 635- 642 ,(1996) , 10.1007/BF00172497
J. Øyaas, I. Storrø, D. W. Levine, Uptake of lactose and continuous lactic acid fermentation by entrapped non-growing Lactobacillus helveticus in whey permeate Applied Microbiology and Biotechnology. ,vol. 46, pp. 240- 249 ,(1996) , 10.1007/S002530050811
A. Ricciardi, E. Parente, G. Addario, Influence of pH on growth and bacteriocin production by Lactococcus lactis subsp. lactis 14ONWC during batch fermentation Applied Microbiology and Biotechnology. ,vol. 41, pp. 388- 394 ,(1994) , 10.1007/BF01982525
S. Benthin, U. Schulze, J. Nielsen, J. Villadsen, Growth energetics of Lactococcus cremoris FD1 during energy-, carbon-, and nitrogen-limitation in steady state and transient cultures Chemical Engineering Science. ,vol. 49, pp. 589- 609 ,(1994) , 10.1016/0009-2509(94)85006-2
E Parente, C Brienza, A Ricciardi, G Addario, Growth and bacteriocin production by Enterococcus faecium DPC1146 in batch and continuous culture Journal of Industrial Microbiology & Biotechnology. ,vol. 18, pp. 62- 67 ,(1997) , 10.1038/SJ.JIM.2900368
Hocine Daba, Christophe Lacroix, Jun Huang, RonaldE. Simard, Influence of growth conditions on production and activity of mesenterocin 5 by a strain of Leuconostoc mesenteroides Applied Microbiology and Biotechnology. ,vol. 39, pp. 166- 173 ,(1993) , 10.1007/BF00228601