Tolerance Mechanism of the Ethanol-Tolerant Mutant of Sake Yeast

摘要: Several ethanol-tolerant mutants have been bred from industrial sake yeasts, but the mechanism of ethanol tolerance in these has not elucidated. After determination entire genome sequence Saccharomyces cerevisiae, various methods to monitor whole-gene expression yeast developed. In this study, we used a commercially available nylon membrane on which virtually every gene S. cerevisiae was spotted compare profiles between mutant and its parent investigate mutant. As result, found that several genes were highly expressed only strain. These known be induced cells exposed stresses, such as ethanol, heat, high osmolarity, or at stationary-phase log-phase. mutant, level stress-responsive further increased after exposure ethanol. We also substances catalase, glycerol trehalose may protective roles under stressful conditions accumulated amounts The exhibited resistance other stresses including osmolarity oxidative stress addition tolerance. results indicate exhibits multiple because elevated genes, resulting accumulation substances.