Neutrophil maturation and hypersegmentation promoted in normal bone marrow by a carcinoma-elaborated protein factor.

摘要: In rats with subcutaneously transplanted mammary carcinoma 5A (MC) and 2 to 10-fold elevations in the blood content of mature neutrophils, 30–50% neutrophils showed pronounced hypersegmentation. This phenomenon could be reproduced liquid culture bone marrow cells from normal (tumor-free) animals by 48 hr incubation MC host's serum, or MC-conditioned medium whose activity was attributable an over 50,000 MW protein. The effects vitro, occurring without change total cell number accompanied increases γ-glutamyltranspeptidase (GGT) alkaline phosphatase (AP) activity, included decreases percents progenitors (myeloblasts, promyelocytes, myelocytes, metamyelocytes bands) increase 50% which exhibited obvious Much less if any neutrophil hypersegmentation, no statistically significant decrease immature cells, occurred response several colony stimulating factors (CSFs) tested, although (in addition inducing GGT AP) some CSFs did cause neutrophils. These investigations demonstrate efficacy a MC-elaborated blood-borne protein promote myeloid maturation, describe system for first time hypersegmentation can studied vitro.