Susceptibility of Field-Collected Aedes aegypti (L.) (Diptera: Culicidae) to Bacillus thuringiensis israelensis and temephos

摘要: The susceptibility status of field-collected Aedes aegypti (L.) from a dengue endemic area to Bacillus thuringiensis israelensis (Bti) and temephos was determined. Since August 2007, biweekly ovitrap surveillance (OS) conducted for 12 mo in 2 sites, A & B, Shah Alam, Selangor. Site treated with Bti formulation, VectoBac® WG at 500 g/ha, December 2007 - June 2008 while B subjected routine vector control activities by the local municipality. larvae collected OS both sites were bred until F3 evaluated their susceptibility. pooled according 3 time periods, which corresponded treatment phases site A: November (Bti pre-treatment phase); July September post-treatment phase). Larvae bioassayed against or accordance WHO standard methods. resistant temephos, incipient detected throughout study using diagnostic dosage 0.02 mg/L. LC50 ranged between 0.007040 0.03799 mg/L year sites. Resistance ratios (LC50) indicated that resistance increased time, 1.2 6.7 folds. Ae. 0.08890 0.1814 showing uniform field Bti, spite receiving 18 treatments over period 7 mo. No cross-resistance detected.