Computer aided desk-top scale-up and optimisation of chromatographic processes

摘要: Abstract Further experimental validation of an existing set computer programs, called Simulus, will be presented. This includes predicting the performance recovery immunoglobulin G from a crude, unclarified tissue culture using Prosep-G fluidised bed and amylase crude feedstock Streamline DEAE expanded bed. The mathematical theory for these programs highlighted. It is not possible to obtain analytical solutions sets equations defining mass transfer processes within column its media. In prediction breakthrough, well established coarse numerical solution sufficient achieve good results, however, when attempting predict sharp elution profiles, model fails. A unique discretisation same presented, giving dramatically improved modelling capabilities. validation, amylase/Sephacryl gel filtration chromatography system, gave convincing results.