A new glance on root-to-shoot in vivo zinc transport and time-dependent physiological effects of ZnSO4 and ZnO nanoparticles on plants.

摘要: Understanding nanoparticle root uptake and root-to-shoot transport might contribute to the use of nanotechnology in plant nutrition. This study performed time resolved experiments probe Zn uptake, biotransformation physiological effects on Phaseolus vulgaris (L.). Plants roots were exposed ZnO nanoparticles (40 300 nm) dispersions ZnSO4(aq) (100 1000 mg L−1) for 48 h. Near edge X-ray absorption spectroscopy showed that 40 nm was more easily dissolved by than 300 nm ZnO. It also leaves found as a mixture Zn3(PO4)2 Zn-histidine complex. fluorescence Zn-translocation presented decreasing gradient concentration velocity, it seems radial movement occurs simultaneously axial xylem transport. Below 100 mg L−1, lower stem tissue section served buffer preventing from reaching leaves. Conversely, not observed L−1 ZnSO4(aq). Transcriptional analysis genes encoding metal carriers indicated higher expression levels tonoplast-localized transporters, suggesting mechanism trend accumulate tissues may be associated with an enhanced compartmentalization vacuoles. The photosynthetic rate, transpiration, water conductance impaired treatments.