Prostaglandins participate in the late phase of the vascular response to acetylcholine iontophoresis in humans.

摘要: The participation of prostaglandins (PGs) in the cutaneous vasodilatation to acetylcholine (ACh) applied via iontophoresis is under debate. Using laser Doppler flowmetry, we studied long lasting effect (20 min) iontophoretic application (30 s; 0.1 mA) ACh on human forearm. Experiments were repeated (1) using deionized water instead test current application, (2) after scopolamine treatment inhibit muscarinic cholinergic receptors, and (3) 2 h, 3 days 10 following inhibition PG synthesis with aspirin or a placebo control. Cutaneous vascular conductance (CVC) was calculated at rest (CVC(rest)), peak first 5 min (CVC(peak)), 20 (CVC(20)). minimal CVC (CVC(min)) also determined. response displayed biphasic pattern an early transient (CVC(peak): 62 +/- 8% maximal induced by local heating (MVC)) followed slower (CVC(min): 44 6; CVC(20): 56 5%MVC). itself had no major effect. Scopolamine almost abolished both phases. phase sensitive but not phase. At hour post-aspirin, CVC(peak) 61 10, CVC(min) 26 6 CVC(20) 29 6%MVC. day 3, 53 9, 22 25 4%MVC. 67 47 7 50 8%MVC. Placebo We conclude that PGs participate vasodilator iontophoresis. Previous non-steroidal anti-inflammatory drug treatments must be taken into account when studying