Circulating microRNAs as biomarkers for diffuse myocardial fibrosis in patients with hypertrophic cardiomyopathy

摘要: Circulating microRNAs may represent novel markers for cardiovascular diseases. We evaluated whether circulating miRNAs served as potential biomarkers diffuse myocardial fibrosis in patients with hypertrophic cardiomyopathy (HCM). Cardiac magnetic resonance imaging postcontrast T1 mapping was performed to non-invasively quantify HCM who were classified into two groups (T1 < 470 ms or T1 ≥ 470 ms, likely unlikely have fibrosis, respectively). First, we screened 84 using human serum/plasma miRNA array on plasma of 8 (4/group based time) and 4 healthy controls. From the results this initial array, 16 selected their fold changes relevance further validation by Taqman real-time PCR 55 patients. Among miRNAs, expression miR-96-5p miR-373-3p low. The remaining 14 (miR-18a-5p, miR-146a-5p, miR-30d-5p, miR-17-5p, miR-200a-3p, miR-19b-3p, miR-21-5p, miR-193-5p, miR-10b-5p, miR-15a-5p, miR-192-5p, miR-296-5p, miR-29a-3p, miR-133a-3p) upregulated T1 < 470 ms compared those 11 (except miR-296-5p significantly inversely correlated values. Individual had moderate diagnostic value (AUC: 0.663–0.742), but greatly improved 0.87) a combination miRNAs. In comparison, collagen turnover did not predictive values fibrosis. These findings suggest that provide attractive candidates putative HCM.