5-Fluorouracil spares hemopoietic stem cells responsible for long-term repopulation.

摘要: The long-term immunohemopoietic reconstituting ability of bone marrow, treated with a single administration 5-fluorouracil (5-FU), was measured to determine whether 5-FU caused any deleterious effect upon primitive stem cells (PSCs). Cells from 5-FU-treated marrow donors were mixed in four different proportions total contents untreated competitor containing genetically distinguishable hemoglobin (Hb) and glucosephosphate isomerase (GPI) transplantation markers. These cell mixtures introduced into lethally irradiated hosts. functional the donor population assessed by measuring percentage type Hb GPI found host's circulating erythrocytes lymphocytes, respectively. Bone mice 1, 5, 8 days prior produced lymphoid erythroid as well equal fractions fresh when surveyed approximately 90 after transplantation. Normal reconstitutive thus maintained despite tenfold reduction numbers had been 5 Donor 15 slightly decreased repopulating one two experiments. A second round repopulation stimulated subsequent initial 90-day screening giving hosts sublethal (500 rad) dose irradiation. After 3-4 months, parameters same preirradiation values. Thus, radioresistance PSCs remains comparable that their relative not comprised additional stress both rounds myeloid pathways repopulated equally surviving treatment. Repopulation extent suggests common precursor proliferative agent. results indicate unaffected treatment 5-FU, although they concentrated tenfold.