Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling.

摘要: The search for new TB drugs that rapidly and effectively sterilize the tissues are thus able to shorten duration of chemotherapy from current 6 months has been hampered by a lack understanding metabolism bacterium when in 'persistent' or latent form. Little is known about condition which bacilli survive, although laboratory models have shown Mycobacterium tuberculosis can exist non-growing, drug-resistant state may mimic persistence vivo. Using nutrient starvation, we established model M. arrests growth, decreases its respiration rate resistant isoniazid, rifampicin metronidazole. We used microarray proteome analysis investigate response starvation. Proteome 6-week-starved cultures revealed induction several proteins. Microarray enabled us monitor gene expression during adaptation starvation confirmed changes seen at protein level. This provided evidence slowdown transcription apparatus, energy metabolism, lipid biosynthesis cell division addition stringent other genes play role maintaining long-term survival within host. Thus, generated with agents active against persistent number potential targets expressed under these conditions.