Immunobiology of β 2 -Microglobulin-Deficient Mice

摘要: The ability to disrupt the function of a given protein molecule is powerful technique. This approach has been extremely useful in modern biology. Monoclonal antibodies have used molecular vitro and vivo. In addition, synthetic antisense oligonucleotides disrupting genes at pretranslational level. depletion particular cell types by treatment with monoclonal complement played major role elucidating activities cells expressing appropriate target molecules. Combinations these approaches led definition lymphocyte subsets their unequivocal characterization vivo vitro. However, precise functions molecules much more difficult attain. prokaryotes lower eukaryotes, produce null mutations important defining numerous gene products, ultimately allowing determination each intact organism. Until recently, it proved obtain mutants mammals. Although there are examples spontaneous and, principle, such can be readily selected vitro, relatively little information derived from these.