Towards the validation of endogenous steroid testing in wildlife hair

摘要: Hair analysis is emerging as a popular tool to examine stress and reproduction hormone levels in wild mammals. The reliability of this approach, however, depends on an understanding steroid incorporation into hair well appropriate validations. We reviewed studies that have examined hormones wildlife with the goal summarizing analytical, physiological, biological evidence approach meaningful. Accordingly, we differentiated among validations aimed at evaluating analytical method versus those designed assess whether reflect physiologically meaningful processes target species. Our literature survey revealed endogenous steroids been from 40 species nonhuman animals across seven mammalian classes. Although majority (85%) 72 reported method, physiological only for five Moreover, results were inconsistent studies. This highlights need further research, carefully differentiate between multiple purported models different types growth patterns. To complement our review published studies, present new data supporting positive relationship steroid, cortisol, blood eight In addition, novel laboratory‐based study showing variable genetically identical laboratory mice kept under controlled conditions. Synthesis applications. Collectively, Review reveals substantial progress towards validation assays variety Further reproductive steroids, combined validations, would expand potential applications analyses research. As key example, can provide mechanistic insights species’ responses change may therefore contribute conservation planning.