Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano.
作者： Magda Grudniewska , Stijn Mouton , Daniil Simanov , Frank Beltman , Margriet Grelling
DOI: 10.7554/ELIFE.20607
关键词:
摘要: The regeneration-capable flatworm Macrostomum lignano is a powerful model organism to study the biology of stem cells in vivo. As amenable transgenesis, it complements historically used planarian models, such as Schmidtea mediterranea. However, information on transcriptome and markers M. limited. We generated de novo assembly performed first comprehensive characterization gene expression proliferating lignano, represented by somatic cells, called neoblasts, germline cells. Knockdown selected set neoblast genes, including Mlig-ddx39, Mlig-rrm1, Mlig-rpa3, Mlig-cdk1, Mlig-h2a, confirmed their crucial role for functionality neoblasts during homeostasis regeneration. signatures will be valuable resource future molecular studies lignano.
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