Water-soluble pristine C60 fullerene attenuates acetaminophen-induced liver injury

摘要: Introduction: Oxidative stress has been suggested as the main trigger and pathological mechanism of toxic liver injury. Effects powerful free radical scavenger С60 fullerene on rat injury cells (HepG2 line) were aimed to be discovered. Methods: Acute (ALI) was simulated by single acetaminophen (APAP, 1000 mg/kg) administration, a chronic CLI, 4 weekly APAP administrations. Pristine C60 aqueous colloid solution (C60FAS; initial concentration 0.15 mg/mL) administered per os or intraperitoneally at dose 0.5 mg/kg 0.25 (CLI) daily for 2 28 days, respectively, after first dose. Animals sacrificed 24th hour last Biochemical markers blood serum autopsies analyzed. EGFR expression in HepG2 48-hour incubation with C60FAS assessed. Results: Increase conjugated unconjugated bilirubin (up 1.4-3.7 times), ALT (by 31-37%), AST 18%) non-treated ALI CLI rats observed, suggesting hepatitis (confirmed histological analysis). Liver morphological state (ALI, CLI), (ALI (CLI), α-amylase, creatinine normalized administration both ways, which may indicate its protective impact liver. However, sharply increased animals receiving 12 times compared control), augmentation metabolism. Furthermore, inhibited dose-dependent manner. Conclusion: could partially correct acute injury, however, it not normalize metabolism exposure.