Sex-related spatial kin structure in a spring population of grey-sided voles Clethrionomys rufocanus as revealed by mitochondrial and microsatellite DNA analyses.

摘要: Polymerase chain reaction-directed mitochondria (mt) and microsatellite DNA analyses were performed to examine the kin structure in a spring population of grey-sided voles Clethrionomys rufocanus Hokkaido, Japan. The spatial distribution 81 trapping grid (about 1 ha) was estimated by using catch-mark-release method. samples extracted from toes clipped for individual identification. Maternal lineages unequivocally determined mtDNA haplotypes, as identified nucleotide sequencing control region. Relatedness between individuals based on genotype allele frequencies at several loci. Although uniform within grid, neighbouring females frequently same maternal lineage. values correlated negatively with geographical distances. Combination two molecular markers revealed four clusters closely related, matrilineal population, whereas no such cluster apparent males. present study first demonstrated sex-related natural vole.