Membrane permeability of fructose-1,6-diphosphate in lipid vesicles and endothelial cells.

摘要: Fructose-1,6-diphosphate (FDP) is a glycolytic intermediate which has been used an intervention in various ischemic conditions for two decades. Yet whether FDP can enter the cell under constant debate. In this study we examined membrane permeability of artificial bilayers and endothelial cells. To examine passive diffusion through bilayer, L-alpha-phosphatidylcholine from egg yolk (Egg PC) (10 mM) multi-lamellar vesicles were created containing different external concentrations (0, 0.5, 5 50 mM). The into was followed spectrophotometrically. results indicate that diffuses bilayer dose-dependent fashion. movement Egg PC confirmed by measuring conversion to dihydroxyacetone-phosphate formation hydrozone. or encapsulated multilamellar placed solution aldolase. mM groups there significant increase dihydroxyacetone/hydrazone indicating crossed intact. We theorized might be due disruption bilayer. hypothesis, small unilamellar composed presence 60 carboxyfluorescein, leakage sequestered dye upon addition FDP, fructose, fructose-6-phosphate, fructose-1-phosphate These increasing rate carboxyfluorescein. contrast, no concentration resulted any could pass cellular membranes, uptake 14C-FDP cells cultured hypoxia normoxia 4 16 h. both treated cells, accompanied loss viability. Our demonstrate diffuse manner.