CD4+FOXP3+ Regulatory T Cells Exhibit Impaired Ability to Suppress Effector T Cell Proliferation in Patients with Turner Syndrome

摘要: Objective We investigated whether the frequency, phenotype, and suppressive function of CD4+FOXP3+ regulatory T cells (Tregs) are altered in young TS patients with 45,X karyotype compared to age-matched controls. Design Methods Peripheral blood mononuclear from (n = 24, 17.4–35.9 years) healthy controls 16) were stained various Treg markers characterize their phenotypes. Based on presence thyroid autoimmunity, categorized into (–) 7) (+) 17). Tregs sorted for CD4+CD25bright co-cultured autologous CD4+CD25− target anti-CD3 -CD28 antibodies assess function. Results Despite a lower frequency CD4+ (-) (mean 30.8% 31.7%, vs. 41.2%; P 0.003 < 0.001, respectively), both groups exhibited higher FOXP3+ among (means 1.99% 2.05%, 1.33%; 0.029 0.004, respectively). There no differences expression CTLA-4 expressing CXCR3+, CCR4+CCR6+ three groups. However, ability suppress vitro proliferation was significantly impaired (P 0.041). Meanwhile, had frequencies naive 0.001 both) but effector memory 0.004 0.002) than did control group. Conclusions The could not efficiently cells, despite increased peripheral cells.