mRNA blood expression patterns in new-onset idiopathic pediatric epilepsy

摘要: Summary Purpose:  Determine if blood messenger RNA (mRNA) expression patterns in children with newly diagnosed untreated idiopathic epilepsy are different from those healthy controls. the differential between patients generalized onset or partial seizures compared to controls. Methods:  Whole was obtained otherwise pediatric along mRNA isolated and hybridized Affymetrix HGU 133 2.0+ microarrays. Analysis performed using Genespring. Differentially expressed gene lists resulted comparison of (1) control groups (2) seizure type subgroups Tissue ontology analysis DAVID. Key Findings:  Thirty-seven 28 controls were included. Overall, 575 genes differentially subjects The subgroup versus (GvC) list (PvC) (p < 0.05). identified almost half GvC PvC as brain based. Functional group several biologically relevant pathways. In GvC, these included mitochondria lymphocyte activation. PvC, we apoptosis, inflammatory defense, cell motion pathways. Significance:  A unique, meaningful pattern is detectable whole new-onset epilepsy. This finds many similar pathways studies examining lesional intractable Blood show promise a target for biomarker development