Interactions between meningeal cells and astrocytes in vivo and in vitro

摘要: Abstract At the interface between meninges and central nervous system there is a characteristic structure known as glia limitans, consisting of many fine interdigitating astrocyte processes which contain both GFAP vimentin, basal lamina. A similar set up after brain injury where meningeal cells invade lesion. We have experimentally put astrocytes in contact with one another, vivo vitro, to see whether this results formation limitans. Cultured were injected into hippocampus adult rats, from 1 12 weeks later brains stained for vimentin. One week injection was widespread astrocytic reaction stretching 2 mm injection, being intensely Over next 4–6 subsided, only remaining vimentin astrocytes, apart those at normal cells, or had migrated needle track. In vitro reminiscent limitans formed patches abutted cells; layer all running parallel two cell types, heavy staining laminin fibronectin. conclude that forms wherever come contact.