Use of oxytocin to attain cervical dilation for transcervical embryo transfer in sheep.

摘要: The aim of this work was to determine whether a cervical dilation protocol (CDP) composed only oxytocin can be used perform transcervical (non-surgical) embryo transfer in sheep (NSET) without affecting the viability corpus luteum (CL). Likewise, we evaluated transposing test with Hegar dilator (CT test), performed at oestrous time, could screen ewes for NSET (greater or lower chances transpose cervix). For that, and ovulation synchronization 25 Santa Ines induce dioestrous condition. Animals went through following CDP crossover design: E + OX, oestradiol benzoate (100 µg intravenously [IV]) IU IV); SAL, saline solution (IV). Using dilator, attempts were (D0) time (D8). CL (morphology, luteal blood flow progesterone values) by ultrasonography (colour Doppler B-mode) serum measurement from D7 D13. rate SAL group (64%; 16/25; p .05) OX (84%; 21/25, > groups. No treatment affected viability. CT showed high sensitivity (85.7%-93.3%), satisfactory accuracy (72%-84%), low false-negative (6.7%-14.6%), but false-positive (46%-66.7%). In conclusion, exclusively lead good rates does not affect CL. addition, screening Hegar) oestrus identify which will likely occur NSET.