Complex of hydrogel with magnetic immobilized GOD for temperature controlling fiber optic glucose sensor
作者： Jun Huang , Peipei Zhang , Mengshi Li , Pengfei Zhang , Liyun Ding
DOI: 10.1016/J.BEJ.2016.07.012
关键词:
摘要: Abstract A fiber optic glucose biosensor based on Poly(N-isopropylacrylamide-co-acrylamide) (P(NIPAAm-co-AAm))-magnetic-immobilized oxidase (GOD) complex (PMIGC)was fabricated to perform the controllable detection of by changing temperature. The was prepared combining P(NIPAAm-co-AAm) with GOD immobilized Fe 3 O 4 @SiO 2 (F)@meso-SiO nanoparticles using in-situ method. Since has lower critical solution temperature (LCST) 36 °C, at above LCST, shrank and PMIGC could not catalyze oxidation glucose. At below swelled catalysis occurred. Because this sensing characteristics PMIGC, can be carried out biosensor. optimal conditions for were achieved pH6.5, 35 °C 12 mg amount. 25 °C, a good linear relationship between Ͽ , phase delay change sensor head, concentration in range 50⿿700 mg/dL (2.78⿿38.89 mmol/L) observed, limit 8.33 mg/dL (0.46 mmol/L) (S/N = 3). This graph defined equation y = 0.01463 + 0.0003313x, R = 0.9914. repeatability selectivity, used practical samples.
sci-hub.se 参考文章(41)
Jun Huang, Huichao Liu, Peipei Zhang, Pengfei Zhang, Mengshi Li, Liyun Ding, Immobilization of cholesterol oxidase on magnetic fluorescent core-shell-structured nanoparticles Materials Science and Engineering: C. ,vol. 57, pp. 31- 37 ,(2015) , 10.1016/J.MSEC.2015.07.038
Zongbing Li, Xiangmin Miao, Aihua Zhu, Liansheng Ling, Hybridization chain reaction and gold nanoparticles dual signal amplification for sensitive glucose detection Biochemical Engineering Journal. ,vol. 103, pp. 205- 210 ,(2015) , 10.1016/J.BEJ.2015.08.001
Mohammad Javad Chaichi, Mahjoobeh Ehsani, A novel glucose sensor based on immobilization of glucose oxidase on the chitosan-coated Fe3O4 nanoparticles and the luminol–H2O2–gold nanoparticle chemiluminescence detection system Sensors and Actuators B-chemical. ,vol. 223, pp. 713- 722 ,(2016) , 10.1016/J.SNB.2015.09.125
T. Sato, K. Katayama, T. Arai, T. Sako, H. Tazaki, Simultaneous determination of serum mannose and glucose concentrations in dog serum using high performance liquid chromatography. Research in Veterinary Science. ,vol. 84, pp. 26- 29 ,(2008) , 10.1016/J.RVSC.2007.03.002
Jipei Yuan, Weiwei Guo, Erkang Wang, Quantum dots-bienzyme hybrid system for the sensitive determination of glucose. Biosensors and Bioelectronics. ,vol. 23, pp. 1567- 1571 ,(2008) , 10.1016/J.BIOS.2008.01.008
I. Delfino, M. Portaccio, B. Della Ventura, D.G. Mita, M. Lepore, Enzyme distribution and secondary structure of sol–gel immobilized glucose oxidase by micro-attenuated total reflection FT-IR spectroscopy Materials Science and Engineering: C. ,vol. 33, pp. 304- 310 ,(2013) , 10.1016/J.MSEC.2012.08.044
Jian-Tao Zhang, Thomas F. Keller, Rahila Bhat, Bora Garipcan, Klaus D. Jandt, A novel two-level microstructured poly(N-isopropylacrylamide) hydrogel for controlled release Acta Biomaterialia. ,vol. 6, pp. 3890- 3898 ,(2010) , 10.1016/J.ACTBIO.2010.05.009
Mihaela Avadanei, Ovidiu Avadanei, Gheorghe Fundueanu, Effect of comonomer ratio and ionic strength on the thermo-induced conformational changes in N-isopropylacrylamide based copolymers: An ATR-FTIR spectroscopic study Vibrational Spectroscopy. ,vol. 61, pp. 133- 143 ,(2012) , 10.1016/J.VIBSPEC.2012.03.001
Bo Wang, Xiao-Ding Xu, Zong-Chun Wang, Si-Xue Cheng, Xian-Zheng Zhang, Ren-Xi Zhuo, Synthesis and properties of pH and temperature sensitive P(NIPAAm-co-DMAEMA) hydrogels. Colloids and Surfaces B: Biointerfaces. ,vol. 64, pp. 34- 41 ,(2008) , 10.1016/J.COLSURFB.2008.01.001
Xian-Zheng Zhang, Yi-Yan Yang, Tai-Shung Chung, Kui-Xiang Ma, Preparation and characterization of fast response macroporous poly(N-isopropylacrylamide) hydrogels Langmuir. ,vol. 17, pp. 6094- 6099 ,(2001) , 10.1021/LA010105V