Regulation of chloride secretion across porcine endometrial epithelial cells by prostaglandin E2

摘要: The endometrial epithelium consists of a surface which faces the uterine lumen and glandular invaginates into stroma (Davis & Blair, 1993). endometrium is responsible for transfer nutrients, fluid electrolytes between circulatory system lumen. transport-related activity provides an optimal intrauterine environment implantation embryo development. Previous studies ionic composition from pigs, humans rats have revealed that K+ concentration 4- to 5-fold higher than in plasma, Na+ significantly lower its plasma (Clemetson, Kim, Mallikarjuneswara Wilds, 1972; Iritani, Sato Nishikawa, 1974; Casslen Nilsson, 1984). Regulation concentrations within oviduct fluids appears be important reproductive events including sperm capacitation, acrosomal reaction, sperm-egg fusion developing (Clemetson et al. Boldt, Casas, Whaley, Creazzo Lewis, 1991; Fraser, 1992, 1995). Other functions epithelial cells include regulation luminal pH volume. Active secretion Cl− HCO3− ions has been shown establish driving force regulate intestine (Wenzl, Sjaastad, Weintraud Machen, 1989; MacLeod, Redican, Lembessis, Hamilton Field, 1996) airways (Smith Welsh, 1992). In rabbit, driven by active across (Leese, 1988). Previous using primary cultures human grown on permeable supports demonstrated amiloride-sensitive absorption was accompanied cell monolayers (Matthews, Thomas, Redfern Hirst, with rodent showed short circuit current stimulated forskolin, activator adenylyl cyclase (Rochwerger, Dho, Parker, Foskett Buchwald, 1994; Leung, Wong, Gabriel, Yankaskas Boucher, cultured mouse cells, adrenergic agonists were produce increase Isc consistent anion secretion. This response mediated β-adrenergic receptors blocked apical addition either glibenclamide or diphenylamine 2,2′-dicarboxylic acid (DPC) (Chan, Fong, Chung 1997). These experiments indicate culture are capable both secretion. Prostaglandins produced stromal other species (Alecozay, Harper, Scheken Hanahan, Zhang, Paria Davis, Chen, Yang, Hilsenrath, Le They act endocrine paracrine manner blood flow, vascular permeability menstruation (Ford, Reynolds Magness, 1982; Keys, King Kennedy, 1986; Chaudhuri, Yee Prostaglandins, specifically PGE2, electrolyte transport function rabbit cortical collecting duct (Sakairi Jacobson, 1995), prairie dog gallbladder (Saunders-Kirkwood, Cates Roslyn, 1993) porcine distal colon (Traynor, Brown O'Grady, However, little known about actions prostaglandins cells. Recent amphotericin B-permeabilized tissues PGF2α increased activation channels basolateral membrane (Vetter 1996). aim this study characterize properties investigate effect PGE2 transport.