In Vitro Studies of the Genetically Determined Unresponsiveness to Thymus-Independent Antigens in CBA/N Mice

摘要: The X-chromosome-linked B lymphocyte defect of CBA/N mice has been studied in vitro by comparing the ability (CBA/N × DBA/2)F 1 (X - /X X + /Y) male and female ) spleen cells to respond thymus-independent antigen DNP (or TNP)-AECM-Ficoll. failed generate significant anti-TNP antibody responses DNP- or TNP-AECM-Ficoll, contrast from F which responded normally these T-independent antigens. Spleen almost as well thymus-dependent antigen, TNP-sheep red blood (TNP-SRBC) vitro. Adding reduce response latter DNP-AECM-Ficoll, suggesting that inability was not due active suppression. TNP-SRBC impaired adding high concentrations TNP-AECM-Ficoll indicating mechanism unresponsiveness tolerance induction all TNP-specific precursors. Lymphocytes were capable forming after stimulation with lipopolysaccharide (LPS) concentrations; DNP-AECM-Ficoll had no effect this polyclonal response. lymphocytes bearing only X-chromosome strain thus display a profound cell activation. This functional may represent either an defective be activated antigens absence sub-class