Biologic and molecular characterization of Toxoplasma gondii isolates from pigs from Portugal.
作者： Susana de Sousa , D. Ajzenberg , Nuno Canada , Lina Freire , J.M. Correia da Costa
DOI: 10.1016/J.VETPAR.2005.08.012
关键词:
摘要: Little is known of Toxoplasma gondii infections in animals Portugal. In the present paper, we report first isolation viable T. from pigs Antibodies to were found 52 (15.6%) 333 prior slaughter using modified agglutination test (MAT) at a serum dilution 1:20. Attempts made isolate 37 seropositive pigs. Samples brain and/or heart each pig digested acid pepsin, and bioassayed into mice. Viable was isolated 15 Restriction fragment length polymorphism on products SAG2 locus amplified by PCR microsatellite analysis revealed that 11 isolates Type II four III. The results indicate phenotypically genetically are similar U.S.
elsevier.com
sci-hub.se 参考文章(18)
R.M. Weigel, E.C. Powell, J.P. Dubey, P. Thulliez, C.D. Andrews, Peter Lind, Sensitivity and specificity of various serologic tests for detection of Toxoplasma gondii infection in naturally infected sows. American Journal of Veterinary Research. ,vol. 56, pp. 1030- 1036 ,(1995)
Ricardo Mondragon, Daniel K. Howe, J. P. Dubey, L. David Sibley, Genotypic analysis of Toxoplasma gondii isolates from pigs Journal of Parasitology. ,vol. 84, pp. 639- 641 ,(1998) , 10.2307/3284743
Tovi Lehmann, Douglas H. Graham, Erica Dahl, C. Sreekumar, Fred Launer, Joseph L. Corn, H.Ray Gamble, J.P. Dubey, Transmission dynamics of Toxoplasma gondii on a pig farm. Infection, Genetics and Evolution. ,vol. 3, pp. 135- 141 ,(2003) , 10.1016/S1567-1348(03)00067-4
John C Boothroyd, Michael E Grigg, Population biology of Toxoplasma gondii and its relevance to human infection: do different strains cause different disease? Current Opinion in Microbiology. ,vol. 5, pp. 438- 442 ,(2002) , 10.1016/S1369-5274(02)00349-1
J. P. DUBEY, G. DESMONTS, Serological responses of equids fed Toxoplasma gondii oocysts. Equine Veterinary Journal. ,vol. 19, pp. 337- 339 ,(1987) , 10.1111/J.2042-3306.1987.TB01426.X
Cláudia B. de A. dos Santos, Ângela C.F.B. de Carvalho, Alessandra M.A. Ragozo, Rodrigo M. Soares, Marcos Amaku, Lúcia E.O. Yai, J.P. Dubey, Solange M. Gennari, First isolation and molecular characterization of Toxoplasma gondii from finishing pigs from São Paulo State, Brazil. Veterinary Parasitology. ,vol. 131, pp. 207- 211 ,(2005) , 10.1016/J.VETPAR.2005.04.039
D. Ajzenberg, A. Dumetre, M.-L. Darde, Multiplex PCR for Typing Strains of Toxoplasma gondii Journal of Clinical Microbiology. ,vol. 43, pp. 1940- 1943 ,(2005) , 10.1128/JCM.43.4.1940-1943.2005
J.P. Dubey, Refinement of pepsin digestion method for isolation of Toxoplasma gondii from infected tissues Veterinary Parasitology. ,vol. 74, pp. 75- 77 ,(1998) , 10.1016/S0304-4017(97)00135-0
J.P. Dubey, Validation of the specificity of the modified agglutination test for toxoplasmosis in pigs Veterinary Parasitology. ,vol. 71, pp. 307- 310 ,(1997) , 10.1016/S0304-4017(97)00016-2
H.R. Gamble, J.P. Dubey, D.N. Lambillotte, Comparison of a commercial ELISA with the modified agglutination test for detection of Toxoplasma infection in the domestic pig. Veterinary Parasitology. ,vol. 128, pp. 177- 181 ,(2005) , 10.1016/J.VETPAR.2004.11.019