The hypochromism of helical polynucleotides.
作者： Howard DeVoe , Ignacio Tinoco
DOI: 10.1016/S0022-2836(62)80106-5
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摘要: The hypochromism of the 260 mμ. absorption band helical DNA is its lower absorbance, per base group, compared to absorbance mononucleotides. has been calculated as a function pf composition and sequence. oscillator strengths, transition monopoles moments for π→π* transitions bases with wavelengths 187 276 mμ have from molecular orbital theory u.v. spectra. effect close 200 mμ, make increase increasing guanine + cytosine content. Experimental measurements hypochromism, while they seem show opposite dependence on composition, are not yet detailed enough definite conclusions be made. in vacuum approximated by polarizabilities solvent refractive index. inside helix cause hypochromism; those sugar phosphate groups hyperchromism. net cannot accurately but should little composition. static electric fields negligible.
sci-hub.se 参考文章(14)
Robert C. Warner, Studies on polynucleotides synthesized by polynucleotide phosphorylase. III. Interaction and ultraviolet absorption. Journal of Biological Chemistry. ,vol. 229, pp. 711- 724 ,(1957) , 10.1016/S0021-9258(19)63676-8
P.D. Lawley, Interaction studies with DNA: III. The effect of changes in sodium ion concentration, pH and temperature on the ultra-violet absorption spectrum of sodium thymonucleate Biochimica et Biophysica Acta. ,vol. 21, pp. 481- 488 ,(1956) , 10.1016/0006-3002(56)90185-8
P. Doty, H. Boedtker, J. R. Fresco, R. Haselkorn, M. Litt, SECONDARY STRUCTURE IN RIBONUCLEIC ACIDS. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 45, pp. 482- 499 ,(1959) , 10.1073/PNAS.45.4.482
Ignacio Tinoco, Optical and Other Electronic Properties of Polymers The Journal of Chemical Physics. ,vol. 33, pp. 1332- 1338 ,(1960) , 10.1063/1.1731408
JULIUS MARMUR, PAUL DOTY, Heterogeneity in deoxyribonucleic acids. I. Dependence on composition of the configurational stability of deoxyribonucleic acids. Nature. ,vol. 183, pp. 1427- 1429 ,(1959) , 10.1038/1831427A0
Ignacio Tinoco, Erratum: Optical and Other Electronic Properties of Polymers The Journal of Chemical Physics. ,vol. 34, pp. 1067- 1067 ,(1961) , 10.1063/1.1731643
William Rhodes, HYPOCHROMISM AND OTHER SPECTRAL PROPERTIES OF HELICAL POLYNUCLEOTIDES Journal of the American Chemical Society. ,vol. 83, pp. 3609- 3617 ,(1961) , 10.1021/JA01478A017
Alexander Rich, Ignacio Tinoco, THE EFFECT OF CHAIN LENGTH UPON HYPOCHROMISM IN NUCLEIC ACIDS AND POLYNUCLEOTIDES Journal of the American Chemical Society. ,vol. 82, pp. 6409- 6411 ,(1960) , 10.1021/JA01509A053
E. Fredericq, A. Oth, F. Fontaine, The ultraviolet spectrum of deoxyribonucleic acids and their constituents Journal of Molecular Biology. ,vol. 3, pp. 11- 17 ,(1961) , 10.1016/S0022-2836(61)80003-X
S.G. Laland, W.A. Lee, W.G. Overend, A.R. Peacocke, The ultraviolet absorption of some degraded desoxyribonucleic acids. Biochimica et Biophysica Acta. ,vol. 14, pp. 356- 365 ,(1954) , 10.1016/0006-3002(54)90193-6