Studies on ribosomal RNA structure

摘要: Abstract 1. Crystallizable RNA fragments, previously obtained by partial alkaline digestion of ribosomes, are a potentially important source information about ribosome structure. The aim the present work was to establish that fragment crystallization is specific indication well-defined structure in rRNA, rather than being an irrelevant artefact. 2. fragments have now been produced treatment isolated rRNA. When alkali used from yeast and rabbit reticulocyte rRNA can be made crystallize, but those Escherichia coli cannot. ribosomal nuclease used, both E. crystallizable. T 1 ribonuclease on crystallizable, when pancreatic they not. subunits ribosomes yield crystallizable fragments. 3. Fragments crystallize only their molecular weights lie approximately range 9000 20 000 (the number chains per particle remains determined). Compared with intact contain less adenine guanine, respectively. This suggests non-helical regions rich purines; such hypothesis would also explain failure results so far support idea derived helical which all types fundamental unit ultimate determine how these interact build up ribosome.