Low-angle x-ray diffraction maxima from ribosomes.

摘要: Abstract 1. Low-angle X-ray diffraction has been recorded from concentrated suspensions of ribosomal particles, mainly yeast 80 S and Escherichia coli 70 50 S. The concentration varied between 4 55%, a range not previously studied. 2. A strong maximum is observed in the 150–350-A range, whose Bragg spacing increases with decreasing concentration. This peak attributable to interparticle interference, showing that particles are behaving as independent, mutually repulsive objects. biological significance this discussed. 3. remaining maxima do change concentration, seem likely arise overall structure individual particles. case for dominant internal repeat 45 weakened by data presented. 4. fit simple model uniform electron density. They might RNA within structure, or sharply defined central hole.