Initiation of Immune Responses in Brain Is Promoted by Local Dendritic Cells

摘要: The contribution of dendritic cells (DCs) to initiating T cell-mediated immune response in and cell homing into the CNS has not yet been clarified. In this study we show by confocal microscopy flow cytometry that expressing CD11c, CD205, MHC class II molecules containing fluorescently labeled, processed Ag accumulate at site intracerebral injection. These follow a specific pattern upon migrating out brain. To track their pathway CNS, differentiated DCs from bone marrow GFP-transgenic mice injected them directly brains naive C57BL/6 mice. We demonstrate migrate brain cervical lymph nodes, process can be blocked fixation or pertussis toxin treatment DCs. Injection OVA-loaded initiates SIINFEKL (a dominant OVA epitope)-specific nodes spleen, as measured tetramer LFA-1 activation marker staining. Additionally, fraction activated SIINFEKL-specific home CNS. Specific however, cannot induced i.v. injection alone. data suggest brain-emigrant are sufficient support tissue DC origination. Thus, initiation reactivity against Ags involves migration APCs nervous peripheral lymphoid tissues, similarly other organs.