An ELISA for apolipoprotein M reveals a strong correlation to total cholesterol in human plasma.
作者： Olof Axler , Josefin Ahnström , Björn Dahlbäck
DOI: 10.1194/JLR.M700113-JLR200
关键词:
摘要: Apolipoprotein M (apoM) is a 188 amino acid, 25 kDa protein belonging to the lipocalin superfamily. Although predominantly associated with high density lipoprotein, apoM found in all major lipoprotein classes. To facilitate clinical studies of apoM, we have developed sandwich ELISA for measurement human plasma. This method has been used investigate normal variation and establish reference values healthy individuals through 598 samples from Nordic Reference Interval Project Bio-bank Database (NOBIDA) biobank. For women 18-49 years old, interval was 0.58-1.18 micromol/l, whereas 50+ men, range 0.61-1.30 micromol/l. Correlation 26 common chemical analytes NOBIDA database revealed marked positive correlation plasma total cholesterol (r = 0.52) LDL HDL 0.43 0.36, respectively). There no statistically significant HDL/total ratio or body mass index. In conclusion, shows that concentration strongly correlated individuals.
sci-hub.se 参考文章(38)
Wolfgang Patsch, Antonio M. Gotto, Apolipoproteins: pathophysiology and clinical implications. Methods in Enzymology. ,vol. 263, pp. 3- 32 ,(1996) , 10.1016/S0076-6879(96)63003-1
George A Mensah, David W Brown, Janet B Croft, Kurt J Greenlund, None, Major coronary risk factors and death from coronary heart disease: baseline and follow-up mortality data from the Second National Health and Nutrition Examination Survey (NHANES II). American Journal of Preventive Medicine. ,vol. 29, pp. 68- 74 ,(2005) , 10.1016/J.AMEPRE.2005.07.030
C.A. Borrebaeck, M.E. Etzler, Production and characterization of a monoclonal antibody against the seed lectin of the Dolichos biflorus plant. Journal of Biological Chemistry. ,vol. 256, pp. 4723- 4725 ,(1981) , 10.1016/S0021-9258(19)69311-7
Rafael A. Cox, Mario R. García-Palmieri, Cholesterol, Triglycerides, and Associated Lipoproteins Butterworths. ,(1990)
E K Harris, J C Boyd, On dividing reference data into subgroups to produce separate reference ranges. Clinical Chemistry. ,vol. 36, pp. 265- 270 ,(1990) , 10.1093/CLINCHEM/36.2.265
Kirsten Faber, Vibeke Hvidberg, Søren K. Moestrup, Björn Dahlbäck, Lars Bo Nielsen, Megalin is a receptor for apolipoprotein M and kidney-specific megalin-deficiency confers urinary excretion of apolipoprotein M. Molecular Endocrinology. ,vol. 20, pp. 212- 218 ,(2006) , 10.1210/ME.2005-0209
A. Smith, J. Esko, S. Hajduk, Killing of trypanosomes by the human haptoglobin-related protein Science. ,vol. 268, pp. 284- 286 ,(1995) , 10.1126/SCIENCE.7716520
W.B. Kannel, J.D. Neaton, D. Wentworth, H.E. Thomas, J. Stamler, S.B. Hulley, M.O. Kjelsberg, Overall and coronary heart disease mortality rates in relation to major risk factors in 325,348 men screened for the MRFIT American Heart Journal. ,vol. 112, pp. 825- 836 ,(1986) , 10.1016/0002-8703(86)90481-3
P. Rustad, P. Simonsson, P. Felding, M. Pedersen, Nordic Reference Interval Project Bio-bank and Database (NOBIDA): a source for future estimation and retrospective evaluation of reference intervals Scandinavian Journal of Clinical & Laboratory Investigation. ,vol. 64, pp. 431- 437 ,(2004) , 10.1080/00365510410006694
Christian Wolfrum, Matthew N Poy, Markus Stoffel, Apolipoprotein M is required for preβ-HDL formation and cholesterol efflux to HDL and protects against atherosclerosis Nature Medicine. ,vol. 11, pp. 418- 422 ,(2005) , 10.1038/NM1211