Method for expressing recombinant human coagulation factor VII and special vector thereof

摘要: The invention discloses a method for expressing recombinant human coagulation factor VII and special vector thereof. From upstream to downstream, the sequentially comprises elongation 1alpha subunit promoter EF-1alpha or cytomegalovirus immediate-early CMV-IE, cDNA (complementary deoxyribonucleic acid) gene of VII, synthetic intron, an internal ribosome entry site IRES, glutamine synthetase GS dihydrofolate reductase DHFR, bovine growth hormone poly A signal sequence plasmid replicating structure domain. detection result expression sample proves that CHO (Chinese hamster ovary) cell transfected by four vectors can stably efficiently express average level is 5-12 mg/L so, has high promotion activity, product shorten time plasma, activity computation indicates maximal equivalent 524% plasma FVII. perform important function in efficient wide application prospects.